and Bio-Dot SF Microfiltration Apparatus - bio-rad.com The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. Many experimental protocols can be accommodated by using interchangeable templates. The Bio-Dot SF apparatus focuses sample to a thin line instead of a circle, making quantitation by densitometry more reproducible. Test Blots, Slot Blots & Dot Blots - Immunodetection | Bio-Rad Each dot or slot blot would contain known amounts of target protein or cell lysate. Once dry, dot blots and slot blots are subjected to the same immunodetection steps used for Western blotting, i.e. blocking, antibody incubation, and target detection with substrate. PROTEIN BIO-DOT (‘SLOT BLOT’)
Dot/Slot (Filtration) Blotting | Life Science Research ...
Western Blot Protocol (8/19/2002). 1.Preparing the sample: -Cutting 20 um frozen sections to a slide, 3-5 of them; depends on the tissue size.Maneuver the gel with forceps or gloved hands to minimize background) -Fill the Bio-Ice cooling unit with water and store it in –20C until ready to use. Anti-RNA blotting with chemiluminescent detection This protocol is for northern blotting/dot-blotting to detect specific RNAs via chemiluminescence, using biotinylated DNA oligo probes.Positively charged nylon membrane (BrightStar-Plus Positively Charged Nylon Membrane, or Roche 11-209-299-001, or Bio-Rad Zeta-Probe #1620154). Western Blot Protocol
Mini Trans-Blot Electrophoretic Transfer Cell Instruction Manual
A Guide to Transfer and Detection - Potts Lab
Protocols. Western Blot Protocol. Reagents Needed: 20X Running Buffer.Open gel cassettes and place the gel on the nitrocellulose membrane sandwiched between two pieces of blotting paper. Place in transfer apparatus and fill with fresh 1X transfer buffer.
Western Blot Theory and Method Guide - SciGine Blotting paper (ProteanXL, #1703966 Bio-Rad). Western Blot Experimental procedure: Unwrap precast gel and rinse wells three times with running buffer.Mix 1 ml each of ECL reagents in a foil-wrapped tube and add to membrane for 5 min prior to imaging on ChemiDoc MP imager ( Bio-Rad). Dot and slot blot hybridization | A learning object for BioMinE… The difference between dot and slot blot procedures is in the way that the nucleic acid mixture is blotted onto the membrane. In dot blotting the nucleic acids are blotted as circular blots, whereas in slot blotting they are blotted into rectangular slots (Figure 1). The latter method allows a more... Western Blot Protocol | Transfer Mini Trans-Blot Cell (…
Bio Rad Dot Blot Manual Microfiltration blotting system, includes dot-blot and slot-blot modules, vacuum manifold base, gasket Bio-Dot Microfiltration Apparatus Instruction Manual. Compared to traditional dot blot, Zoom Plate only needs 1/1000 amount of antibody for each sample, a Conventional protocol
Dot Blot Laboratory Protocol - UF CPET Dot Blot Laboratory Protocol The dot blot is a technique for detecting, analyzing, and identifying proteins, similar to the western blot technique. The dot blot differs from the western in that the protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate. Slot Blot Hybridization Protocol - Tramvia Napoli
Troubleshooting Quantitative Western Blots Hints and Tips If you encounter high background or unexpected bands, try a different blocker. For tips on how to choose an appropriate blocker, get the Western Blot Blocker Optimization for Near-Infrared Detection protocol. Antibody cross-reactivity in a two-color Western blot. In two-color Western blots, antibody cross-reactivity is always a possibility. Dot and slot blotting of DNA - ResearchGate Dot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative ... Mini-PROTEAN II Electrophoresis Cell Instruction Manual Bio-Rad also recommends that all Mini-PROTEAN II cell components and accessories be cleaned with a suitable laboratory cleaner (such as Bio-Rad Cleaning Concentrate, catalog number 161-0722) and rinsed thoroughly with distilled water before use. WARNING: Do not use alcohol to clean the plastic parts of the Mini-PROTEAN II cell.